Fig 2: BMSC colony formation and phenotypes. (A) Colony formation was observed with using magnifications of ×10 and ×100. (B) In order to investigate the characterization of the BMSCs, the expression of the antigens on the cell surface were detected using flow cytometry. Results demonstrated that the harvested cells were positive for mesenchymal progenitor cell-like markers CD44 and CD90, but were negative for the hematopoietic marker CD45. BMSC, bone mesenchymal stem cell; CD, cluster of differentiation; PE, phycoerythrin; FITC, fluorescein isothiocyanate.

Fig 3: BMSC culture identification and Lv-BMP-7 transfection. (a) Morphological observation and identification of rat BMSCs (100x). Flow cytometry was used to detect CD29 and CD44 on the cell surface. The red peak was a negative control, and the green peak was a positive expression peak. (b) Lv-BMP-7 transfection efficiency identification with different MOIs (100x).

Fig 4: Comparison of multipotency of hair follicle stem cells treated with different growth media. Flow cytometric analysis of key mesenchymal stem cell surface markers revealed, that almost all cells in the three experimental groups expressed CD44 and CD90, but not haematopoietic markers (CD34 and CD45).

Fig 5: Identification of BMSCs. Morphological images (×100 magnification) of BMSCs cultured continuously for (A) 7 and (B) 14 days. BMSCs at 4 passages were positive for (C) oil red O staining (×200 magnification) and (D) ALP staining (×400 magnification) after 3 weeks of osteogenesis induction. (E) BMSCs surface markers expressed CD29, CD44 and CD90, and were negative for hematopoietic cell surface antigen markers CD34 and CD45.